Is RNA Synthesis Possible Without Transcription Bubble Formation?
نویسندگان
چکیده
منابع مشابه
RNA displacement and resolution of the transcription bubble during transcription by T7 RNA polymerase.
Unlike DNA polymerases, RNA polymerases (RNAPs) must displace the nascent product from the template and restore the DNA to duplex form after passage of the transcription complex. To accomplish this, RNAPs establish a locally denatured "bubble" that encloses a short RNA:DNA hybrid. As the polymerase advances along the template, the RNA is displaced at the trailing edge of the bubble and the two ...
متن کاملFunctional transcription elongation complexes from synthetic RNA-DNA bubble duplexes.
A synthetic RNA-DNA bubble duplex construct intended to mimic the nucleic acid framework of a functional transcription elongation complex was designed and assembled. The construct consisted of a double-stranded DNA duplex of variable length (the template and nontemplate strands) containing an internal noncomplementary DNA "bubble" sequence. The 3' end of an RNA oligonucleotide that is partially...
متن کاملIs Science Without Spacetime Possible?
Numerous approaches to a quantum theory of gravity posit fundamental ontologies that exclude spacetime, either partially or wholly. This situation raises deep questions about how such theories could relate to the empirical realm, since arguably only entities localized in spacetime can ever be observed. Are such entities even possible in a theory without fundamental spacetime? How might they be ...
متن کاملSynthesis of RNA by in vitro transcription.
In vitro transcription is a simple procedure that allows for template-directed synthesis of RNA molecules of any sequence from short oligonucleotides to those of several kilobases in μg to mg quantities. It is based on the engineering of a template that includes a bacteriophage promoter sequence (e.g. from the T7 coliphage) upstream of the sequence of interest followed by transcription using th...
متن کاملWhat is Possible Without Disturbing Quantum Signals?
Consider a situation in which a quantum system is secretly prepared in a state chosen from the known set of n states. We show that the fundamental properties of classical and quantum signals lead to a principle that gives a definite distinction between the operations that preserve the states of the system and those that do not. Many types of no-cloning and no-imprinting conditions can easily be...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Sciences
سال: 2006
ISSN: 1727-3048
DOI: 10.3923/jbs.2006.532.539